Fluorescence barrier filter

The role of the barrier filter is to block any reflected excitation light and transmit only the fluorescence from the subject. The barrier filter is positioned in front of the viewing device, whether that is your eye or any kind of camera - film, digital, or video. The photographs below show what the barrier filter does.

The NightSea barrier filters are available in either glass or plastic. The glass barrier filters are typically provided in standard photographic filter mounts, as outlined on the photographic products page. With plastic we can provide the filter material in a very wide range of shapes and sizes. We can also add holes to aid in attaching custom filter sizes to your equipment, or you can drill and shape the material yourself. For custom cuts the price depends on the size of the filter requested, and the complexity of any machining we need to do. Contact NightSea for a quote.

All images © Justin Marshall and Charles Mazel This photograph was made with white light with no exciter filter and no barrier filter. That is, it is a 'normal' reflected-light photograph.   This photograph was made with the NightSea blue exciter filter in place, but no barrier filter. The fluorescence of the border appears pink and the center blue-green, and blue light is reflected from all other surfaces.   This photograph was made with both the blue exciter filter and yellow barrier filter in place. This is a 'proper' fluorescence photograph. All of the reflected blue light from the excitation source has been blocked from the camera and we now see the orange fluorescence of the border and the green fluorescence of the center. Two green-fluorescent spots are evident to the right of the main specimen.   All images © Justin Marshall and Charles Mazel

NightSea
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