Hirox microscope with NIGHTSEA fluorescence adapter

Fluorescence Adapter for Hirox Digital Microscope

The NIGHTSEA Model SFA Fluorescence Adapter system can add a versatile fluorescence imaging capability to the Hirox digital microscope. Visit our gallery of images made with the NIGHTSEA system and the Hirox microscope.

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Fluorescence solutions

The key elements of any fluorescence system are:

  • a light source that produces sufficient energy in the appropriate wavelength range to excite fluorescence in the sample of interest;
  • a barrier filter in the viewing path that blocks reflected excitation light while transmitting the fluorescence emitted by the sample.

NIGHTSEA implements these for the Hirox microscope with:

  • high intensity LED light sources available in five excitation wavelength ranges (see list further down page);
  • emission barrier filters that can be added to the Hirox lenses easily and non-invasively.

(Click image for larger view)


Here is a sample of what you can see. For many more examples visit our Hirox image gallery.


Comprehensive solution kit

We have configured a versatile Hirox fluorescence adapter kit that includes the most useful excitation wavelengths for varied industry applications. The SFA-H-IND kit includes the following items, at a package price that is significantly less than purchasing the items separately:

  • Three excitation light heads – Ultraviolet (UV), Violet (VI), and Royal Blue (RB)
    • This recommendation is based on our experience with varied applications. Read the explanation below.
  • Three barrier filters paired to the excitation light heads
  • Matching barrier filter glasses for each excitation wavelength
  • Gooseneck lamp base with dimming control
  • Universal power supply – 120/240VAC, 50/60Hz, with international plug set
  • Packing/carrying case with custom-cut foam

We do offer additional wavelength sets that may be of use for other applications.

Use this Quote Request Form to request a quotation for the SFA-H-IND kit or for any other configuration.


Why we recommend a set of three excitation wavelengths for industry needs

We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:

  • A prospective customer wanted to image concrete thin sections impregnated with fluorescent dye. The dye supplier recommended UV, and it is true that UV will make the dye fluoresce nicely. We found that our Royal Blue excitation produced significantly brighter fluorescence.
  • Similar to above, except that a fluorescence epoxy had been used to highlight surface cracks in a test sample. Royal Blue excitation yielded brighter fluorescence.
  • A prospective customer sent us a sample of material that was known to fluoresce under UV. The goal was to see the distribution of non-fluorescent particles in a fluorescent matrix. While both UV and Royal Blue excited fluorescence in the sample, the best imaging contrast was achieved with Violet excitation.

Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact NIGHTSEA If you would like to discuss your application further.


Wavelength sets

There are five excitation/emission wavelength combinations available for the system.

Color Designation Excitation Emission
Ultraviolet UV 360 – 380nm 415nm longpass
Violet VI 400 – 415nm 450nm longpass
Royal Blue RB 440 – 460nm 500nm longpass
Cyan CY 490 – 515nm 550nm longpass
Green GR 510 – 540nm 600nm longpass