Fluorescence Images by Steve Ackleson

The images on this page were frame-grabbed from underwater fluorescence video taken by Dr. Steven Ackleson, with the assistance of Charlie Mazel. This was our first trial of using the Underwater Kinetics Light Cannon with the NightSea exciter filter as a video light for fluorescence. Steve operated the camera and Charlie held the light. For some of the shots Steve turned on his conventional filament video lights so we could acquire reflected-light images for comparison.

Technical details:

Location: Mile Marker 4, Kona Coast, Hawaii

Camera: Sony PD-150

Housing: Sea & Sea

Camera filter: NightSea barrier filter custom mountedoutside lens port

Light source: Underwater Kinetics Light Cannon

Excitation filter: NightSea BE1

Exposure and focus: automatic

In addition to the frame-grabbed video below we have three AVI video sequences you can download for viewing.

Video 1, 2.8 MB

Video 2, 1.5 MB

Video 3, 273 kB

All images © Steve Ackleson       White light and fluorescence of the same subject White light and fluorescence of the same subject White light and fluorescence of the same subject White light and fluorescence of the same subject White light and fluorescence of the same subject. Note the dead branches in the middle of the colony. They lack the green fluorescent pigment that is in the living coral and are completely dark in the fluorescence image. The sequence of 4 images above shows a transition from all reflected to all fluoresced light   All images © Steve Ackleson

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