A custom NIGHTSEA Eclipse MicroTent™ enables a clinical hematology laboratory to perform a sensitive fluorescence-based blood analysis in their well-lit space.
A hematology laboratory in a hospital had an ongoing workflow problem. One of their significant blood test procedures is based on fluorescence, and while they owned an appropriate microscope the blood lab is a large, open, well-lit room (Figure 1). Fluorescence microscopy is typically done in darkened areas, and they could not see the fluorescence from their very small targets reliably because of the strong ambient light.
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When a patient is suspected of being infected with the parasites associated with either malaria (transmitted by mosquitoes) or babesiosis (transmitted by ticks), a blood sample is sent to the laboratory. The sensitive Quantitative Buffy Coat (QBC) method is used for the screening. QBC involves centrifugation of the blood in the presence of a fluorescent stain that attaches to the parasites. A fluorescence microscope is then used to examine the sample.
The laboratory’s Olympus Model BH2 compound fluorescence microscope with the BH2-RFL light source sat unused. In its place they used the same model microscope owned by another group in the hospital. That microscope was in a dimly lit area that made the assay possible, but there were some significant downsides – they could only use the equipment on a non-interference basis; the other group was on a different floor; and the microscope was inside a Biosafety Level 2 (BSL-2) negative pressure room used for tuberculosis testing, requiring special preparation just to enter the room. This all combined to make it an inconvenient arrangement that got in the way of a smooth workflow.
The lab was considering renovating a storage closet or building an enclosure within their space just to house their microscope, and they were also searching for less intrusive (and expensive) solutions. Technicians at the laboratory learned about the NIGHTSEA Eclipse MicroTent™, a patented (US Pat. No. 10,175,467) ‘dark tent’ solution for shielding microscopes from ambient light and approached us see if our product could assist them.
Our standard MicroTent™ is made for small stereo microscopes and would not fit around the lab’s compound microscope. We had already been thinking about ways to be more versatile in providing custom solutions and decided to take this project on as a test case. To start the process the lab sent us detailed photographs from various angles with a tape measure in place. One important consideration was that the light source is a 100W mercury vapor bulb and the lamp housing and the optic tube leading to the microscope get very hot (Figure 2).
We designed a new enclosure tailored to the Olympus BH-2 (Figure 3) and then collaborated with the lab to test prototypes and iterate to a final form. The light-restricting material is a true blackout fabric that is supported by a framework of plastic tubing and elbows custom cut for the project. The microscope eyepieces project through a flexible sleeve on the top of the enclosure. The design leaves the hot lamp housing and optic tube outside the tent. The front of the enclosure lifts up to allow easy access to the microscope stage and to the positioning and focusing controls (Figure 4). The tent prevents interfering ambient light from reaching the sample, and the included NIGHTSEA Tru-Block Eye Shields block light from the user’s peripheral vision (Figure 5).
With the tent in place laboratory personnel can now perform QBC tests at their convenience. The laboratory director wrote:
“Thank you for solving an operational workflow dilemma! The dark tent will be helpful for performing microscopy while saving the expense of a dark room and increasing the testing portability.”
Do you need a custom darkness solution for your microscopy? Contact NIGHTSEA for more information.