The NIGHTSEA Model SFA Fluorescence Adapter system adds a versatile fluorescence imaging capability to the Keyence VHX series of digital microscopes. The system is being used successfully by a growing number of Keyence owners for a variety of applications. Visit our gallery of images made with the NIGHTSEA adapter and the Keyence microscope.
Keyence offers a variety of lenses for imaging at different scales. NIGHTSEA offers several variants of our fluorescence adapter system that work with the most commonly used Keyence lenses – the ZST, Z100, Z50, Z20, and Z00.
The key elements of any fluorescence system are:
NIGHTSEA implements these for the Keyence system with:
(Click image for larger view)
Here is a sample of what you can see. For many more examples visit our Keyence image gallery.
We have configured a versatile Keyence fluorescence adapter kit (part number SFA-K-IND) that includes the most useful excitation wavelengths for varied industry applications, and that adapts to all of the supported Keyence lenses. The SFA-K-IND kit includes the following items, at a package price that is significantly less than purchasing the items separately:
We do offer additional wavelength sets that may be of use for other applications.
Use this Quote Request Form to request a quotation for the SFA-K-IND kit or for any other configurations.
We have found that the combination of the Ultraviolet, Violet, and Royal Blue excitation/emission sets addresses a wide variety of industrial imaging challenges. The most common misconception we encounter is that fluorescence is uniquely associated with ultraviolet (UV) light and many customers immediately ask for that wavelength. However we have found in several cases – even some in which UV was specifically recommended by a dye manufacturer – that either Violet or Royal Blue provided superior performance. For example:
Acquiring the three-color system will provide the highest chance of success and will equip you with a versatile toolkit to address new fluorescence imaging challenges as they arise. Contact NIGHTSEA If you would like to discuss your application further.
The microscopes in the Keyence VHX series were not designed for fluorescence. That said, the NIGHTSEA adapter system adds a fluorescence capability that is proving to be valuable for a growing number of users. Several companies have purchased multiple adapters so that they could replicate the performance across multiple microscopes. In at least one case the customer only purchased their Keyence system because of the added NIGHTSEA fluorescence capability that enabled a critical analysis function for them.
Read more about performance expectations with the NIGHTSEA adapters on the Keyence microscopes.
There are five excitation/emission wavelength combinations available for the system.
|Ultraviolet||UV||360 – 380nm||415nm longpass|
|Violet||VI||400 – 415nm||450nm longpass|
|Royal Blue||RB||440 – 460nm||500nm longpass|
|Cyan||CY||490 – 515nm||550nm longpass|
|Green||GR||510 – 540nm||600nm longpass|
With the ZST lens and the higher magnification (200 – 2000x) objective you can achieve enhanced performance by removing the Keyence fiber optic illuminator and positioning the NIGHTSEA excitation light head in its place with our custom adapter. This brings the excitation energy through the lens, creating a much more concentrated illumination spot than can be achieved with the light source positioned to the side. We have made fluorescence images at up to 2000x with this configuration.
Read more about achieving the best fluorescence results with the ZST lens.