Fluorescent Zebrafish – NIGHTSEA Stereo Microscope Fluorescence Adapter

These images of fluorescent transgenic zebrafish and the video were made while demonstrating the Stereo Microscope Fluorescence Adapter system at the Connecticut Valley Zebrafish Meeting at Williams College in September 2014. The zebrafish are expressing cmlc:GFP (heart) and gata1:dsRed (blood cells). Note that you can see the blood cells even with the Royal Blue excitation that is the best choice for GFP. Zebrafish courtesy of Dr. Martha Marvin (Williams College), transgenic line bred by Dr. Lara Hutson (University of Buffalo).

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The image set below shows fish under white light, blue light excitation, and green light excitation

In the following set we have added a little white light to the blue-light-excited image to provide more context.