New Green Bandpass Filter for the Stereo Microscope Fluorescence Adapter

Posted On: Tuesday, August 19, 2014

Arabidopsis fluorescence imaged with bandpass filter (c) NIGHTSEA

We are now offering an additional barrier filter option for the Stereo Microscope Fluorescence Adapter. The new filter isolates the green part of the spectrum and is for use with the Royal Blue excitation source. While our other barrier filters are long-pass filters this new filter is a bandpass, transmitting from approximately 500 to 560nm. The long-pass filter has served well for most users who need to visualize green-fluorescent protein (GFP), and if you are exploring fluorescence in nature it is preferable. A primary motivation for adding this green-only filter to the line-up was for the benefit of researchers using GFP in plants such as Arabidopsis thaliana, a common research model. Plants contain chlorophyll, which has a distinctive red fluorescence that can sometimes mask the GFP emission, making it harder to see and photograph. The filter can also be useful for other viewing situations in which background autofluorescence reduces viewing contrast.

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