Posted On: Monday, February 29, 2016
The NIGHTSEA Stereo Microscope Fluorescence Adapter (SFA) improved workflow for research with mouse embryos by detecting GFP-positive specimens and aiding in dissection – right in the mouse facility.
For investigation of the physiological properties of motor and sensory neurons a research group collects spinal cords from d12 – d13 mouse embryos carrying a particular trait. Only 50% of the offspring carry the trait, and the trait-positive mice express GFP in the spinal cord. Their normal procedure was to harvest embryos in the mouse facility and dissect out all of the spinal cords. Only then can they bring them to another area of the building to screen them under a fluorescence microscope and discard the unneeded samples. This process resulted in a lot of wasted effort. The group was looking for a better way to select the GFP-positive embryos immediately post-collection and pre-dissection without leaving the mouse facility.
The solution – Use the gooseneck illuminator that comes with the SFA to do double duty. Using it as a desk lamp in combination with a pair of barrier filter glasses the team can screen all of the embryos at once immediately after collection. They can then dissect the GFP-positive specimens under the stereo microscope, aided by the fluorescence.