Calcein fluorescence detection

Posted On: Tuesday, February 10, 2015

Calcein-stained goby imaged with BlueStar light (c) Greg Andraso and Kelly Grant, Gannon U


Researchers are using NIGHTSEA’s flashlights and microscope adapters are the preferred visualization tools for studies with fish stained with calcein. When fish are immersed in a calcein solution it is incorporated into calcified structures such as otoliths, fin rays, vertebrae, and scales.

Calcein is under evaluation as a tool for mass marking fish for release/recapture fisheries studies, and several groups are successfully using the BlueStar flashlight and filter glasses to distinguish marked from unmarked individuals. Other researchers are utilizing calcein for laboratory studies and using the Stereo Microscope Fluorescence Adapter for high resolution observation and imaging.

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