Fluorescence microscopy – NIGHTSEA with Unitron and Zeiss

Posted On: Friday, March 25, 2016

Zeiss 508 - ostracod

I (NIGHTSEA founder Charlie Mazel) recently worked on fluorescence microscopy with Maureen Donahue, proprietor of MD Micro Solutions, an independent microscope dealer based in New England. We were reviewing our Model SFA Stereo Microscope Fluorescence Adapter and its accessories, and trying the system out with a variety of subjects on different microscope/camera combinations:

The SFA system worked great with both setups. Below are some sample pictures.

(Click any image for larger view)

Fluorescence microscopy with Zeiss system

Maureen had collected a sample from the shore in Gloucester, Massachusetts. The images below show fluorescence using our Royal Blue excitation in combination with the longpass filter.

The first image is an intact barnacle shell. The deep red fluorescence arises from chlorophyll, while the orange is from phycoerythrin, a photosynthetic accessory pigment in the blue-green algae. The second and third images show a barnacle, the animal itself, with a broken shell. When feeding the barnacle extends its fan-like appendage into the water column to capture its prey. The last image is an ostracod, a small bivalve crustacean about the size of a grain of sand.

Fluorescence microscopy with Unitron system

The images below are of nylon granules with a fluorescent defect called ‘gel’. Fluorescence is used for quality control in nylon granule manufacturing.

This next set of images is more related to the forensic sciences:

  • Dust bunny, imaged under white light and fluorescing under Ultraviolet and Royal Blue excitation
  • Small fragment of a finger bone in dirt
  • Anti-counterfeiting strip on a US $20 bill



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